Preparation of an immunoaffinity chromatography column for chloramphenicol binding
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چکیده
منابع مشابه
Preparation of Knockout Extract by Immunoaffinity Column and Its Application
Importance of herbal medicines have recently increased owing to rising interest in their health benefits. However, medicinal plant extracts are complex mixtures of phytochemicals that act synergistically or additively on specific and/or multiple molecular and cellular targets. Thus, it is difficult to examine the actual pharmacological roles of active compounds in plant extracts. This review de...
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Affinity chromatography is a high resolution, high capacity, and one of the most powerful and diverse methods for separating proteins and other biological molecules of interest on the basis of a highly specific, reversible biological interaction between two molecules: an affinity ligand attached to a solid matrix to create a stationary phase, and a target molecule in a mobile phase. Specificall...
متن کاملIsolation and concentration of Salmonellae with an immunoaffinity column.
A method for rapidly and selectively isolating Salmonellae from buffer solutions and concentrating the bacteria by a factor of approximately 500 was developed. Anti-Salmonellae antibody was covalently linked to 40 microm polyacrylamide beads to prepare a solid phase with affinity for the bacteria. The beads were packed into 1-mm diameter glass tubes to form a column 20 microl in volume. Buffer ...
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ژورنال
عنوان ژورنال: Science and Technology Development Journal
سال: 2016
ISSN: 1859-0128,1859-0128
DOI: 10.32508/stdj.v19i4.688